Phone: (302) 831-3449
Office: DBI 117A
Phone: (302) 831-0162
Office: DBI 122
Zeiss 880 at Wolf Hall - Confocal / Spectral / Airyscan
The LSM 880 microscope is a fully automated multimodal microscope that can be used for confocal and spectral imaging. The Airyscan detector enables super-resolution and high speed imaging.
The LSM880 is fully equipped for live-cell imaging. It has an environmental enclosure (temperature, humidity, and CO2 control) and z-drift correction to maintain samples at the focal plane over time.
-Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy.
-Super-resolution imaging. The Airyscan can provide a 2-fold increase in resolution compared to conventional confocal microscopy.
-High-speed imaging. The Airyscan detector can be used to image 4 times faster than the confocal mode (up to 20fps at 512*512). The 880 is also equipped with a piezo-electric stage for high-speed acquisition of z-stacks.
-Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography.
-FRET, FRAP, FLIP.
-Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions.
-Tile scanning to image a large field at high resolution.
-Live cell imaging thanks to the environmental enclosure.
Location: Wolf Hall #304
Contact person: Dr. Le Marchand
Leica 3050 Cryostat at Wolf Hall
The Leica CM3050 cryostat is used to cut sections of frozen samples embedded in a tissue freezing medium.
Location: Wolf Hall #331
Contact persons: Deborah Powell or Jean Ross