Confocal Microscopy


Zeiss 880 at DBI - Confocal / Spectral / Multiphoton / Airyscan

The LSM 880 microscope is a fully automated multimodal microscope that can be used for confocal, spectral, and multiphoton excitation imaging. The Airyscan detector enables super-resolution and high speed imaging.

The LSM880 is fully equipped for live-cell imaging. It has an environmental enclosure (temperature, humidity, and CO2 control) and z-drift correction to maintain samples at the focal plane over time.

 

Applications:

-Confocal imaging.

-Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy.

-Multiphoton excitation microscopy. It can be used to image up to 10x deeper than confocal microscopy.

-Super-resolution imaging. The Airyscan can provide a 2-fold increase in resolution compared to conventional confocal microscopy.

-High-speed imaging. The Airyscan detector can be used to image 4 times faster than the confocal mode (up to 20fps at 512*512). The 880 is also equipped with a piezo-electric stage for high-speed acquisition of z-stacks.

-Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography.

-FRET, FRAP, FLIP.

-Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions.

-Tile scanning to image a large field at high resolution.

-Live cell imaging thanks to the environmental enclosure.

Objectives: 10x/0.3, 20x/0.8, 40x/1.2 W, 40x/1.3 Oil, 63x/1.4 Oil – more objectives are available as add-ons.

Laser lines: 405nm, 458nm, 488nm, 514nm, 561nm, 633nm, 680-1080nm.

Detectors: a 32-channel ultra-sensitive spectral GaAsP PMT (Gallium Arsenide Phosphide PhotoMultiplier Tube) detector (i.e. ChS in Zen) which is ideal for spectral imaging, two multi-alkali PMT detectors (Ch1 & Ch2), one transmitted PMT (T-PMT) for contrast imaging (Bright Field, Differential Interference Contrast, Phase), and one GaAsP Airyscan detector for super-resolution and fast imaging.

Operating software: Zen 2.3 SP1

Location: DBI 117C

Contact person: Dr. Le Marchand

 

Zeiss 880 at Wolf Hall - Confocal / Spectral / Airyscan

The LSM 880 microscope is a fully automated multimodal microscope that can be used for confocal and spectral imaging. The Airyscan detector enables super-resolution and high speed imaging.

The LSM880 is fully equipped for live-cell imaging. It has an environmental enclosure (temperature, humidity, and CO2 control) and z-drift correction to maintain samples at the focal plane over time.

Applications:

-Confocal imaging.

-Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy.

-Super-resolution imaging. The Airyscan can provide a 2-fold increase in resolution compared to conventional confocal microscopy.

-High-speed imaging. The Airyscan detector can be used to image 4 times faster than the confocal mode (up to 20fps at 512*512). The 880 is also equipped with a piezo-electric stage for high-speed acquisition of z-stacks.

-Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography.

-FRET, FRAP, FLIP.

-Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions.

-Tile scanning to image a large field at high resolution.

-Live cell imaging thanks to the environmental enclosure.

 

Objectives: 5x/0.25, 10x/0.3, 20x/0.8, 40x/1.2 W, 40x/1.3 Oil, 63x/1.4 Oil.

Laser lines: 405nm, 458nm, 488nm, 514nm, 561nm, 594nm, 633nm.

Detectors: a 32-channel ultra-sensitive spectral GaAsP PMT (Gallium Arsenide Phosphide PhotoMultiplier Tube) detector (i.e. ChS in Zen) which is ideal for spectral imaging, two multi-alkali PMT detectors (Ch1 & Ch2), one transmitted PMT (T-PMT) for contrast imaging (Bright Field, Differential Interference Contrast, Phase), and one GaAsP Airyscan detector for super-resolution and fast imaging.

Operating software: Zen 2.3 SP1

Location: Wolf Hall #304

Contact person: Dr. Le Marchand

Zeiss 780 Multiphoton Confocal

The Zeiss LSM 780 is an upright confocal (objectives are above the sample) that enables confocal, spectral, and multiphoton excitation imaging. The non-descanned detection system can be used for two-photon excitation fluorescence or detection of second harmonic generation (SHG) in the forward and reverse directions. The 780 is particularly well-suited for intravital imaging of small animals.

Applications:

-Confocal imaging.

-Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy.

-Multiphoton excitation microscopy. It can be used to image up to 10x deeper than confocal microscopy. The upright configuration, non-descanned detection system and the Z-deck add-on are ideal for intravital imaging of small animals. The system is also ideal for reverse and forward detection of second harmonic generation (e.g. can be used to image collagen fibers).

-Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography.

-FRET, FRAP, FLIP.

-Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions.

-Tile scanning to image a large field at high resolution.

Objectives: 10x/0.3, 20x/0.75, 40x/1.2 W, 40x/1.4 Oil. Other objectives available in the room: 20x/0.6, 20x/1 W, 40x/0.8 W, 63x/0.9 W, 63x/1.4 Oil.

Laser lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm, 690-1040nm.

Detectors: a 32-channel ultra-sensitive spectral GaAsP PMT (Gallium Arsenide Phosphide PhotoMultiplier Tube) detector (i.e. ChS in Zen), two multi-alkali PMT detectors (Ch1 & Ch2), two non-descanned GaAsP PMT detectors, and one transmitted PMT (T-PMT) for contrast imaging (Bright Field, Differential Interference Contrast, Phase).

Operating software: Zen 2.3 SP1

Location: CBBI (Center for Biomedical and Brain Imaging) #244C

Contact person: Dr. Le Marchand

Zeiss 710 Confocal/Spectral

The LSM 710 microscope is a fully automated microscope that can be used for confocal and spectral imaging. The system is fully equipped for live-cell imaging. It has an environmental enclosure (temperature, humidity, and CO2 control) and z-drift correction to maintain samples at the focal plane over time.

Applications:

-Confocal imaging.

-Spectral imaging with linear unmixing. It can be used to remove background autofluorescence or for multicolor imaging when spectral overlaps between colors cannot be separated with standard confocal microscopy.

-Confocal reflection microscopy. It can be used to image collagen fibers and surface microtopography.

FRET, FRAP, FLIP.

-Fluorescence Correlation Spectroscopy imaging. It provides quantitative data on concentrations, diffusion coefficients, molecular transport and interactions.

-Tile scanning to image a large field at high resolution.

-Live cell imaging thanks to the environmental enclosure.

Objectives: 10x/0.3, 20x/0.75, 40x/1.2 W, 40x/1.4 Oil. Other objectives available in the room: 20x/0.6, 20x/1 W, 40x/0.8 W, 63x/0.9 W, 63x/1.4 Oil.

Laser lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 594nm, 633 nm.

Detectors: a 32-channel spectral multi-alkali PMT (i.e. ChS in Zen), two multi-alkali PMT detectors (Ch1 & Ch2), two ultra-sensitive GaAsP PMT detectors (Gallium Arsenide Phosphide PhotoMultiplier Tube), and one transmitted PMT (T-PMT) for contrast imaging (Bright Field, Differential Interference Contrast, Phase).

Operating software: Zen 2012 SP5

Location: DBI 117Z

Contact person: Dr. Le Marchand

Zeiss 5 LIVE DUO Highspeed Confocal / Spectral

The Zeiss 5 LIVE DUO is line-scanning microscope and has all of the capabilities of a standard confocal. The high speed DUO detector is optimal for capturing fast cellular dynamics and can collect images at 120 frames per second. The META is a spectral detector that can separate fluorescent compounds with similar spectral profiles.

A stage top incubator is available for live cell imaging.

Laser Lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm

Location: DBI 117Z

Contact person: Dr. Caplan