Super-Resolution Microscopy

Andor Dragonfly - Confocal / TIRF / Super Resolution

The Dragonfly microscope is a fully automated multimodal microscope that can be used for confocal, total internal reflection microscopy (TIRF) and super-resolution (localization-based techniques such as: PALM, dSTORM, PAINT, etc.).

The LSM880 is fully equipped for live-cell imaging. It has a stage top environmental enclosure (temperature, humidity, and CO2 control) and Z-drift correction to maintain samples at the focal plane over time.

Applications:

  • Spinning disk confocal imaging. In contrast to point scanning confocals, such as the LSM880 and CD7 that serially acquire one focal point at a time to reconstitute a 2D image, the Dragonfly acquires confocal images by simultaneously acquiring thousands of spots from the focal plane. This setup enables the Dragonfly to provide several important advantages over point-scanning confocals:
    • Faster speed (up to ~200 frames per second), which is ideal for fast 2D or 3D time-series or tile scanning.
    • Gentler imaging – photobleaching and phototoxicity are greatly reduced, which is better for live cell imaging.
    • The high sensitivity of cameras is well suited for dim specimens.
  • Super-resolution imaging. The Dragonfly can perform 3D localization- based microscopy techniques (dSTORM, PAINT, etc.) that improve spatial resolution to ~30nm laterally, and ~50nm axially.
  • TIRF microscopy. a technique allowing the confined excitation of fluorophores within ~100nm above the coverslip.

Objectives: 10x/0.45, 20x/0.8, 40x/1.1 W, 40x/1.3 Oil, 63x/1.47 Oil.

Laser lines: 405nm, 488nm, 561nm, 638nm, 735nm.

Detectors: Two Andor Zyla 4.2 sCMOS cameras can be used together to acquire two colors simultaneously.

Operating software: Fusion

Location: APB 141R

Contact persons: Dr. Le Marchand (confocal), Tim Chaya (super-resolution)