Andor Dragonfly - Confocal / TIRF / Super Resolution
The Dragonfly microscope is a fully automated multimodal microscope that can be used for confocal, total internal reflection microscopy (TIRF) and super-resolution (localization-based techniques such as: PALM, dSTORM, PAINT, etc.).
The LSM880 is fully equipped for live-cell imaging. It has a stage top environmental enclosure (temperature, humidity, and CO2 control) and Z-drift correction to maintain samples at the focal plane over time.
- Spinning disk confocal imaging. In contrast to point scanning confocals, such as the LSM880 and CD7 that serially acquire one focal point at a time to reconstitute a 2D image, the Dragonfly acquires confocal images by simultaneously acquiring thousands of spots from the focal plane. This setup enables the Dragonfly to provide several important advantages over point-scanning confocals:
- Faster speed (up to ~200 frames per second), which is ideal for fast 2D or 3D time-series or tile scanning.
- Gentler imaging – photobleaching and phototoxicity are greatly reduced, which is better for live cell imaging.
- The high sensitivity of cameras is well suited for dim specimens.
- Super-resolution imaging. The Dragonfly can perform 3D localization- based microscopy techniques (dSTORM, PAINT, etc.) that improve spatial resolution to ~30nm laterally, and ~50nm axially.
- TIRF microscopy. a technique allowing the confined excitation of fluorophores within ~100nm above the coverslip.
Objectives: 10x/0.45, 20x/0.8, 40x/1.1 W, 40x/1.3 Oil, 63x/1.47 Oil.
Laser lines: 405nm, 488nm, 561nm, 638nm, 735nm.
Detectors: Two Andor Zyla 4.2 sCMOS cameras can be used together to acquire two colors simultaneously.
Operating software: Fusion
Location: APB 141R
Contact persons: Dr. Le Marchand (confocal), Tim Chaya (super-resolution)