Mr. Richard West
Specialty: Flow Cytometry, Cell Sorting
Flow Cytometry Core
Center for Biomedical & Brain Imaging (CBBI), Room 244
Newark, DE 19711
My primary expertise is in multicolor flow cytometry and high speed cell sorting. I also have extensive experience in mammalian cell culture (primary, stem cells, cell lines) and hematology. I can provide guidance with all aspects of your experiments, including initial experimental design, sample preparation and technical assistance during flow cytometric acquisition and analysis. I can provide hands on training enabling walk-up usage of core facility instrumentation.
I am interested in the underlying mechanisms that lead to bone marrow failure disorders such as myelodysplastic syndromes, aplastic anemia and leukemia. I also study bone marrow microenvironment/niche with a focus on marrow stromal cells and hematopoiesis. I have worked extensively with immune function and regulation, murine models of leukemia, mouse tumor models, GVHD, hemoglobinopathies, macrophages and antigen presenting cells.
Duggan J, Okonta H, Elnaggar D, French J, West R, Chakraborty J. Retrovirus Induced Lymphomagenesis - A Correlation Between disease pathogenesis and flow cytometric analysis. J Gen Virol. 2012 Jun 6; 93:2028-2036. DOI: 10.1099/vir.0.043661-0
Isaev DA, Garitaonandia I, Abramihina TV, Zogovic-Kapsalis T, West RA, Semechkin AY, Müller AM, Semechkin RA. In vitro Differentiation of Human Parthenogenic Stem Cells Into Neural Lineages. Regen Med. 2012 Jan;7(1):37-45. PMID: 22168496.
Turovets N, Fair J, West R, Ostrowska A, Semechkin R, Janus J, Cui L, Agapov V, Turovets I, Semechkin A, Csete M, Agapova L. Derivation of High Purity Definitive Endoderm from Human Parthenogenic Stem Cells Using and in vitro Analog of the Primitive Streak. Cell Transplant. 2011 Jun 9. PMID: 21669044.
Harris CA, Resau JH, Hudson EA, West RA, Moon C, Black AD, McAllister JP 2nd. Effects of surface wettability, flow, and protein concentration on macrophage and astrocyte adhesion in an in vitro model of central nervous system catheter obstruction. J Biomed Mater Res A. 2011 Apr 11. Doi: 10.1002/jbm.a.33078 PMID: 21484987.
Harris CA, Resau JH, Hudson EA, West RA, Moon C, Black AD, McAllister JP 2nd. Reduction of protein adsorption and macrophage and astrocyte adhesion on ventricular catheters by polyethylene glycol and N-acetyl-L-cysteine. J Biomed Mater Res A. 2011 May 31:98A:425–433 doi: 10.1002/jbm.a.33130. PMID: 21630435.
Zhang YW, Staal B, Essenburg C, Su Y, Kang L, West RA, Kaufman D, Dekoning T, Eagleson B, Buchanan SG, Vande Woude GF. MET Kinase Inhibitor SGX523 Synergizes with EGFR Inhibitor Erlotonib in an HGF-Dependent Fashion to Suppress Carcinoma Growth. Cancer Res. 2010 Jul 19;70(17):6880-6890 10.1158/0008-5472.CAN-10-0898. PMID: 20643778.
Harris CA, Resau JH, Hudson EA, West RA, Moon C, McAllister JP. Mechanical Contributions to Astrocyte Adhesion Using a Novel in vitro Model of Catherter Obstruction. Exp Neurol. 2010 Jan 4;222(2):204-210. DOI:10.1016/j.expneurol.2009.12.027. PMID: 20051241.
DeWard, A.D., K. Leali, R. West, G.C. Prendergast, and A.S. Alberts. In Press. Loss of RhoB Expression Enhances the Myelodysplastic Phenotype of mammalian Diaphanous-related Formin mDia1 Knockout Mice. PLoS One. 2009 Sep 21;4(9):e7102.PMID: 19768111.
Eisenmann KM, Dykema KJ, Matheson SF, Kent NF, DeWaard AD, West RA, Tibes R, Furge KA, Alberts AS. 5q– Myelodysplastic Syndromes: Chromosome 5q Genes Direct a Tumorsuppression Network Sensing Actin Dynamics. Nature Oncogene. 2009 Oct 1;28(39):3429-3441.
Lindvall C, Zylstra CR, Evans N, West RA, Dykema K, Furge KA and Williams BO. The Wnt CoReceptor Lrp6 is Required for Normal Mouse Mammary Development. PLoS ONE. 2009 Jun 5;4(6):e5813.
Knudsen BS, Zhao P, Resau J, Cottingham S, Gherardi E, Xu E, Resau J, Berghuis B, Daugherty J, Grabinski T, Toro J, Giambernardi T, Skinner RS, Gross M, Hudson E, Kort E, Lengyel E, Ventura A, West RA, Xie Q, Hay R, Woude GV, Cao B. A Novel Multipurpose Monoclonal Antibody for Evaluating Human c-Met Expression in Preclinical and Clinical Settings. Appl Immunohistochem Mol Morphol. 2009 Jan;17(1): 57–67. doi:10.1097/PAI.0b013e3181816ae2
Eisenmann KM, West RA, Hildebrand D, Kitchen SM, Peng J, Sigler R, Zhang J, Siminovitch KA, & Alberts AS. T cell Responses in Mammalian Diaphanous-related Formin mDia1 Knockout Mice. J. Biol. Chem, 2007 Jun 26;282:25152-25158. 10.1074/jbc.M703243200
Peng J, Kitchen SM, West RA, Sigler R, Eisenmann KM, & Alberts AS. Myeloproliferative Defects Following the Targeting of the Drf1 Gene Encoding the Mammalian Diaphanous-related Formin mDia1. Cancer Res. 2007 Aug 15;67(16):7565-7571
Mageed AS, Pietryga DW, DeHeer DH, West RA. Isolation of Large Numbers of Mesenchymal Stem Cells From the Washings of Bone Marrow Collection Bags: Characterization of Fresh Mesenchymal Stem Cells. Transplantation. 2007 Apr 27;83(8):1019-1026
Sheehan KM, DeLott LB, West RA, Bonnema JD, DeHeer DH. Hyaluronic acid of high molecular weight inhibits proliferation and induces cell death in U937 macrophage cells. Life Sci. 2004:75;26:3087-3102