Confocal Microscopy


 Zeiss 5 LIVE DUO Highspeed/ Spectral Confocal

The Zeiss 5 LIVE DUO has all of the capabilities of a standard confocal and two specialized detectors. The high speed DUO detector is optimal for capturing fast cellular dynamics and can collect images at 120 frames per second. The META is a spectral detector that can separate fluorescent compounds with similar spectral profiles.

Specifications:

  • Laser Lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
  • Special Features:
    • High Speed
    • Spectral Imaging
    • Stage Top Incubator

Location: DBI 117Z

 

Zeiss 880 Confocal

The LSM880 multiphoton confocal microscope has been optimized for live cell imaging.  With a full environmental enclosure (temperature, humidity, and CO2 control) and hardware (autofocus) it is optimized for long, live-cell time course experiments. It is also significantly faster than the other confocal microscopes and can run up to 13 frames per second.  The detectors are similar to the LSM780, but the liquid cooling and optimized scanning results in a significant increase in the signal-to-noise ratio.

Location: DBI 117C

Zeiss 710 Confocal

The new illumination and detection design of LSM 710 brings complete freedom to your fluorescence microscopy. You work with up to ten dyes and use continuous spectral detection across the complete wavelength range. LSM 710 enables confocal microscopy for a wide variety of applications. With the inverted Axio Observer, the LSM 710 offers you unrivaled confocal microscopy in cell and developmental biology. 

Location: DBI 117Z

Zeiss 780 Multiphoton Confocal

The Zeiss LSM 780 is an upright confocal with 32 channel ultra sensitive GaAsP detectors that allow visualization of weak fluorescent signals with lower photobleaching and phototoxicity. In addition, the system can be used for spectral imaging, photon counting and fluorescence correlation spectroscopy (FCS). The added Multiphoton laser is a 3 watt Chameleon Vision II with a range of 680 - 1080 nm. The NDD detection system can be used for imaging multiphoton florescence or detection of second harmonic generation (SHG)in the forward and reverse directions. Furthermore, the new OSCiscan function allows faster acquisition of high quality data and better time resolution.

Specifications:

  • Laser Lines: 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, 633 nm
  • Special Features:
    • Upright Microscope
    • Chameleon Vision II Multiphoton Laser
    • Reverse and Forward NDD
    • Fluorescence Correlation Spectroscopy (FCS)
    • Spectral Imaging
    • Z-Deck Add-On for Intravital Imaging
    • Photon Counting

Location: Wolf Hall 304