Dr. Jeffrey L. Caplan
Specialty: Confocal Microscopy and Super-resolution Microscopy
Director of Bio-Imaging
University of Delaware
15 Innovation Way, Suite 117
Newark, DE 19711
My primary expertise is in confocal microscopy techniques, which includes point scanning confocal microscopy, multi-photon microscopy, spectral confocal microscopy, and high-speed line scanning confocal microscopy. I can provide guidance with all aspects of your experiments, including initial experimental design, sample preparation and technical assistance during imaging. Furthermore, I can assist with advanced techniques, such as environmentally controlled live-cell imaging, FRAP, FRET, BiFC, photoactivation, 4D imaging and image processing. In addition to confocal microscopy, I have expertise in conventional light microscopy, laser capture microdissection and cryo scanning electron microscopy.
My research focuses on employing advanced microscopy techniques to study the function chloroplast extension during programmed cell death (PCD) and plant innate immunity. Chloroplasts connect to nuclei via tubule-like extensions called stromules. I am investigating how stromules form and function duringchloroplasts-to-nuclearcommunication during PCD and plant innate immunity.
Gangadharan, V., Nohe, A., and Caplan, J. (2014). Caveolin-1 Regulates P2X 7 Receptor Signaling in Osteoblasts. J Physiol Cell Physiol.
Scheiblin, D. a, Gao, J., Caplan, J.L., Simirskii, V.N., Czymmek, K.J., Mathias, R.T., and Duncan, M.K. (2014). Beta-1 integrin is important for the structural maintenance and homeostasis of differentiating fiber cells. Int. J. Biochem. Cell Biol. 1–14.
Warner, C. a., Biedrzycki, M., Jacobs, S.S., Wisser, R.J., Caplan, J.L., and Sherrier, D.J. (2014). An Optical Clearing Technique for Plant Tissues Allowing Deep Imaging and Compatible with Fluorescence Microscopy. Plant Physiol. 166, 1684–1687.
Lazouskaya, V., Wang, L.-P., Or, D., Wang, G., Caplan, J.L., and Jin, Y. (2013). Colloid mobilization by fluid displacement fronts in channels. J. Colloid Interface Sci. 406, 44–50.
Mathioni, S.M., Patel, N., Riddick, B., Sweigard, J. a, Czymmek, K.J., Caplan, J.L., Kunjeti, S.G., Kunjeti, S., Raman, V., Hillman, B.I., et al. (2013). Transcriptomics of the rice blast fungus Magnaporthe oryzae in response to the bacterial antagonist Lysobacter enzymogenes reveals candidate fungal defense response genes. PLoS One 8, e76487.
Naik, M.U., Caplan, J.L., and Naik, U.P. (2013). Junctional adhesion molecule-A suppresses platelet integrin αIIbβ3 signaling by recruiting Csk to the integrin-c-Src complex. Blood 1393–1402.
Nandety, R.S., Caplan, J.L., Cavanaugh, K., Perroud, B., Wroblewski, T., Michelmore, R.W., and Meyers, B.C. (2013). The role of TIR-NBS and TIR-X proteins in plant basal defense responses. Plant Physiol. 162, 1459–1472.
Kumar, A.S., Lakshmanan, V., Caplan, J.L., Powell, D., Czymmek, K.J., Levia, D.F., and Bais, H.P. (2012). Rhizobacteria Bacillus subtilis restricts foliar pathogen entry through stomata. Plant J. 72, 694–706.
Lakshmanan, V., Kitto, S.L., Caplan, J.L., Hsueh, Y.-H., Kearns, D.B., Wu, Y.-S., and Bais, H.P. (2012). Microbe-associated molecular patterns-triggered root responses mediate beneficial rhizobacterial recruitment in Arabidopsis. Plant Physiol. 160, 1642–1661.
Wang, C., Fuller, M.E., Schaefer, C., Caplan, J.L., and Jin, Y. (2012). Dissolution of explosive compounds TNT, RDX, and HMX under continuous flow conditions. J. Hazard. Mater. 217-218, 187–193.
Young, C.L., Raden, D.L., Caplan, J.L., Czymmek, K.J., and Robinson, A.S. (2012). Cassette series designed for live-cell imaging of proteins and high-resolution techniques in yeast. Yeast 119–136.
Wei, D., Jacobs, S., Modla, S., Zhang, S., Young, C.L., Cirino, R., Caplan, J., and Czymmek, K. (2012). High-resolution three-dimensional reconstruction of a whole yeast cell using focused-ion beam scanning electron microscopy. Biotechniques 53, 41–48.
Caplan, J., Niethammer, M., Taylor, R.M., and Czymmek, K.J. (2011). The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional. Curr. Opin. Struct. Biol.21, 686–693.
Huang, K., Czymmek, K.J., Caplan, J.L., Sweigard, J.A., and Donofrio, N.M. (2011a). Suppression of plant-generated reactive oxygen species is required for successful infection by the rice blast fungus. Virulence 2, 559–562.
Huang, K., Czymmek, K.J., Caplan, J.L., Sweigard, J. a, and Donofrio, N.M. (2011b). HYR1-mediated detoxification of reactive oxygen species is required for full virulence in the rice blast fungus. PLoS Pathog. 7, e1001335.
Zhu X, Caplan J, Mamillapalli P, Czymmek K, Dinesh-Kumar SP (2010). Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death. EMBO 5, 1007-1018.PMCID :PMC2837167
CaplanJ.L, ZhuX.H., Mamillapalli P., Marathe R., Anandalakshmi R. and Dinesh-KumarS.P. (2009). Induced ER-chaperones regulate a novel receptor-like kinase to mediate a viral innate immune response. Cell Host and Microbe 5, 457-69.PMCID: PMC2784700
Caplan, J.L., Mamillapalli, P., Burch-Smith, T.M., Czymmek, K., and Dinesh-Kumar, S.P. (2008). Chloroplastic Protein NRIP1 Mediates Innate Immune Receptor Recognition of a Viral Effector. Cell 132, 449-462. PMCID: PMC2267721
Burch-Smith, T.M., Schiff, M., Caplan, J.L., Tsao, J., Czymmek, K., and Dinesh-Kumar, S.P. (2007).A Novel Role for the TIR Domain in Association with Pathogen-Derived Elicitors.PLoSBiol 5, e68. PMCID: PMC1820829