Zeiss Axioplan 2 Upright Light Microscope
The Zeiss Axioplan 2 is an upright epi-fluorescence microscope. It is capable of brightfield, fluorescence, phase contrast, darkfield, differential interference contrast (DIC) and Z stacks. The system can be fitted with one of two color cameras depending on imaging needs. The AxioCam color camera is only used for Z stack collection using the AxioVision 4.8 software. All other digital imaging uses the Axiocam 512 color camera with ZEN Blue 2.3 Pro software.
Cameras:
- Axiocam 512
- AxioCam
Operating Software:
- ZEN Blue 2.3 Pro
- AxioVision 4.8
Fluorescent Filter Sets:
- Blue/DAPI
- Green/eGFP
- Red/Cy3
Objectives:
- 10X Acroplan/0.25, Ph1
- 20x Plan-Apochromat/0.75
- 40x Plan-Neofluor/0.75, Ph2
- 40x LD Achroplan/0.6
- 63x Plan-Apochromat/1.4, oil, Ph3, DIC
Location: 141Q
Zeiss Axio Zoom Stereo Microscope
The Zeiss Axio Zoom.V16 stereo microscope combines a 16x zoom with a high numerical aperture, NA 0.25. The system is capable of both brightfield and fluorescence imaging. Superior brightness can be observed as well as viewing complete model organisms. Digital imaging is acquired with a color camera.
Camera: Axiocam 512
Operating Software: ZEN Blue 2.3 Pro
Fluorescent Filter Sets:
- Blue/DAPI
- Green/GFP
- Red/RFP
Objectives:
- 1x PlanNeoFluar Z/0.25
- 2.3x PlanNeoFluar Z/0.57
Location: 141Q
Zeiss M2BIO Dissecting Microscope
The Zeiss M2BIO is a stereo-microscope equipped with fluorescent filters for DAPI, FITC, and Rhodamine. The AxioCam HR color CCD can be mounted on the microscope for image acquisition using the AxioVision software.
Location: APB 141H
Zeiss PALM MicroBeam Laser Capture Microdissection Microscope
The Zeiss PALM MicroBeam Laser Capture Microdissection (LCM) Microscope is a dual use system.
- As an LCM, it uses a pulsed solid-state 355nm (UV-A) laser for isolating specific cells for downstream analysis. It can isolate DNA, RNA, proteins, chromosomes, single cells, small organisms and live cells. Samples for LCM can be viewed in either brightfield or fluorescence mode. The LCM can isolate samples on slides or in culture dishes. Supporting substrates can be either standard glass microscope slides or special membrane slides (PEN or PET slides). PET membrane slides are ideal for fluorescence work. The LCM is compatible with both cryostat-sectioned or formalin fixed and paraffin embedded tissue. The Palm Robo software is used for LCM collection.
- The system can also be utilized as an inverted microscope for both brightfield and fluorescent imaging. Advanced imaging techniques such as tile sets, Z stacks and time series are capable using the ZEN Blue software.
Cameras:
- Color: Axiocam 503 color
- Fluorescent: Axiocam 503 mono
Operating Software:
- PALM Robo 4.8
- ZEN Blue 2.6 Pro
- AxioVision SE64 4.9.1
Fluorescent Filter Sets:
- Blue/DAPI
- Green/FITC
- Red/TRITC
- Far Red/Cy5
Objectives:
- 5x Fluar/0.25
- 10x Fluar/0.5, DIC II
- 20x LD PlanNeofluar/0.4, DIC II
- 40x LD PlanNeofluar/0.6, DIC II
- 63x LD PlanNeofluar/0.75, DIC II
- 100x PlanNeofluar/1.3, Oil, DIC III
Location: 141Q